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Senior Lecturer in Molecular Genetics | Email: | n.crickmore@sussex.ac.uk |
| Phone: | +44 1273 678917 / 678897 | |
| Fax: | +44 1273 678433 |
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This bacterium forms the major part of my research interests. It is a Gram positive spore-former that synthesizes a large cytoplasmic crystal containing insecticidal toxins. We are interested in the mechanism of in vivo crystallisation, the mechanism of action of the toxin within the insect gut, and the ways in which insects develop resistance to the toxins. |
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Mosquito ControlIn collaboration with the Corporacion para Investigones Biologicas, Colombia and with funding from The Wellcome Trust we have been looking at methods of creating improved biological mosquitocides using recombinant DNA technology. |
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COST 862 - Bacterial toxins for insect controlFurther details of this action, which I chair, can be found here |
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Bacillus thuringiensis Toxin Gene NomenclatureIf you've stumbled across this page whilst looking for the Bt toxin gene
nomenclature site that I maintain, or indeed if you're interested in this
project, it is currently residing here |
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Scientific JournalsI am on the editorial board of two international journals Journal of Invertebrate Pathology Biocontrol Science and Technology I am also the previous chair of the bacterial division of the Society for Invertebrate Pathology |
| Name | Status | Research Area | Funding |
|---|---|---|---|
| Zenas George | DPhil Student | Mosquito control |
Nigerian Government
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| Vidisha Krishnan | DPhil Student | Toxin mechanism of action | |
| Ruchir Misra | MPhil Student | Insecticide resistance | |
| Donghai Peng | Visiting Fellow | Novel insecticides |
BBSRC
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| Alicia Elhigazi | MSc Student | ||
| Elnaz Esfahani | MSc Student | ||
| JingQiu Hu | MSc Student | ||
| Cinthiya Jacob | MSc Student | ||
| Lauren Small | Project Student | ||
| Shraddha Patel | Project Student | ||
| Dan Baldwin | Project Student | ||
| Nicola Bishop | Project Student |
Past Lab Members
| Changlong Shu | Visiting Student | 2009 |
| Goutham Raval | MSc Student | 2008-9 |
| Prashanth Saseedharan | MSc Student | 2008-9 |
| Paul Johnston | DPhil Student | 2006-9 |
| Stella Stamatopoulou | MSc Student | 2007-8 |
| Venkatasamy Balasubramani | Visiting Fellow | 2007-8 |
| Zhang Wenfei | Visiting Student | 2008 |
| Mark Bruce | DPhil Student | 2005-8 |
| Ali Sayyed | Post-doc | 2005-8 |
| Sarfraz Ali Shad | Visiting Student | 2007-8 |
| Ruchir | MSc Student | 2006-7 |
| Munir Ahmad | Visiting Student | 2006-7 |
| Ko-Chun Lou | MSc Student | 2005-6 |
| Vignesh Venkatraman | MSc Student | 2005-6 |
| Dasheng Zheng | Visiting Fellow | 2005-6 |
| Jeremy Coen | Post-doc | |
| Simon Jennings | Post-doc | |
| Roxani Gatsi | DPhil Student | |
| Thaleia Kouskoura | DPhil Student |
Bacillus thuringiensis - an impotent pathogen? (2010)
Ben Raymond, Paul Johnston, Christina Nielsen-LeRoux, Didier Lereclus, Neil
Crickmore
Trends in Microbiology in press
Gut bacteria not required for Bacillus thuringiensis insecticidal activity
towards the tobacco hornworm, Manduca sexta (2009)
Paul Johnston and Neil Crickmore
Applied and Environmental Microbiology 75: 5094-5099
A mid-gut microbiota is not required for the pathogenicity of Bacillus thuringiensis
to diamondback moth larvae (2009)
Ben Raymond, Paul Johnston, Denis Wright, Richard Ellis, Neil Crickmore, Michael
Bonsall
Environmental Microbiology 11, 2556-2563
Genetic Characterisation of Resistance to Deltamethrin in Plutella xylostella
(Lepidoptera: Plutellidae) from India (2008)
Venkatasamy Balasubramani, Ali H. Sayyed and Neil Crickmore
Journal of Economic Entomology 101, 1911-1918
Fitness Costs limit the Development of Resistance to Indoxacarb and Deltamethrin
in Heliothis virescens (Lepidoptera: Noctuidae) (2008)
Ali H. Sayyed, Munir Ahmad and Neil Crickmore
Journal of Economic Entomology 101, 1927-1933
Genetic, Biochemical, and Physiological Characterization of Spinosad Resistance
in Plutella xylostella (Lepidoptera: Plutellidae) (2008)
Ali H. Sayyed, Shafqat Saeed, M Noor-Ul-Ane and Neil Crickmore
Journal of Economic Entomology 101, 1658-1666
Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis
with high toxicity to the Asian corn borer and other lepidopteran insects (2008)
Xue J, Liang G, Crickmore N, Li H, He K, Song F, Feng X, Huang D, Zhang J.
FEMS Microbiology Letters 280, 95-101
Cross-resistance between a Bacillus thuringiensis Cry toxin and non-Bt
insecticides in the diamondback moth. (2008)
Ali H. Sayyed, Graham Moores, Neil Crickmore and Denis J. Wright
Pest Management Science 64, 813-819
Mechanisms of resistance to Bacillus thuringiensis in the diamondback
moth. (2007)
Mark J. Bruce, Roxani Gatsi, Neil Crickmore and Ali. H. Sayyed
Biopesticides International 3, 1-12
Genetics and Mechanism of Resistance to Deltamethrin in a Field Population
of Spodoptera litura (Lepidoptera: Noctuidae). (2007)
Munir Ahmad, Ali H. Sayyed, Neil Crickmore and Mushtaq A. Saleem
Pest Management Science 63, 1002-1010
Selection of a field population of Diamondback Moth (Lepidoptera: Plutellidae)
with acetamiprid maintains, but does not increase, cross-resistance to pyrethroids.(2007)
Ali H Sayyed and Neil Crickmore
Journal of Economic Entomology 100: 932-938
Expression of mosquitocidal Cyt1Aa toxin from Bacillus thuringiensis
subsp. israelensis in Asticcacaulis excentricus (2007)
Zheng Da-sheng, Crickmore Neil, Cai Ya-jun, Yan Jian-ping, YuanZhi-ming
Acta Microbiologica Sinica 47, 217-220
Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus (2006) Dasheng Zheng, Norma Adriana Valdez-Cruz, Gemma Armengol, Chloe Sevrez, Jose Maurilio Munoz-Olaya, Zhiming Yuan, Sergio Orduz, Neil Crickmore (2006). Current Microbiology 54, 58-62
Beyond the spore - past and future developments of Bacillus thuringiensis
as a biopesticide (2006)
Neil Crickmore
Journal of Applied Microbiology 101, 616-619
Common, but complex, mode of resistance of Plutella xylostella toBacillus
thuringiensis toxins Cry1Ab and Cry1Ac (2005)
Ali H. Sayyed, Roxani Gatsi, M. Sales Ibiza-Palacios,Baltasar Escriche, Denis
J. Wright, and Neil Crickmore
Applied and Environmental Microbiology 71, 6863-6869
Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa
in the aquatic bacterium Asticcacaulis excentricus (2005)
Gemma Armengol, Oscar Guevara, Sergio Orduz and Neil Crickmore
Current Microbiology 51, 430-433
Using worms to better understand how Bacillus thuringiensis kills insects
(2005).
Neil Crickmore
Trends in Microbiology 13, 347-350
Structure, diversity and evolution of protein toxins from spore-forming entomopathogenic
bacteria (2003)
Ruud A. de Maagd, Alejandra Bravo, Colin Berry, Neil Crickmore and H. Ernest
Schnepf
Annual Review of Genetics 37, 409-433
Identification of a novel DNA methyltransferase activity from Bacillus thuringiensis
(2003)
Elizabeth Jenkinson and Neil Crickmore
Current Microbiology 47, 144-145
N-terminal activation is an essential early step in the mechanism of action
of the Bacillus thuringiensis Cry1Ac
insecticidal toxin. (2002)
Alejandra Bravo, Jorge Sanchez, Thaleia Kouskoura and Neil Crickmore
Journal of Biological Chemistry 277, 23985-23987
The diverse armoury of the Bt crystal (2002)
Neil Crickmore
Proceedings of the 6th International Conference on Bacillus thuringiensis
pp147-152
ISSN 1516-781X
Cyt1Aa from Bacillus thuringiensis var israelensis is toxic to
the diamondback moth, Plutella xylostella and synergizes
the activity of Cry1Ac towards a resistant strain. (2001)
Ali Sayyed, Neil Crickmore and Denis Wright
Applied and Environmental Microbiology. 67, 5859-5861
Expression and crystallization of an N-terminally activated form of the Bacillus
thuringiensis Cry1Ca toxin. (2001)
Thaleia Kouskoura, Candy Tickner and Neil Crickmore.
Current Microbiology. 43, 371-373
Susceptibility of a field-derived, Bt-resistant strain of diamondback moth
to in vitro activated Cry1Ac toxin. (2001)
Ali Sayyed, Roxani Gatsi, Thaleia Kouskoura, Denis Wright and Neil Crickmore
Applied and Environmental Microbiology. 67, 4372-4373
Cross-resistance and stability of resistance to Bacillus thuringiensis
toxin Cry1C in diamondback moth. (2001)
Yong-Biao Liu, Bruce Tabashnik, Susan Meyer and Neil Crickmore.
Applied and Environmental Microbiology. 67, 3216-3219
How Bacillus thuringiensis has evolved specific toxins to colonize the
insect world. (2001)
Ruud de Maagd, Alejandra Bravo and Neil Crickmore.
Trends in Genetics. 17, 193-199
Cellular Localization and characterization of the Bacillus thuringiensis
Orf2 crystallisation factor. (2001)
Nicholas Staples, David Ellar and Neil Crickmore.
Current Microbiology. 42, 388-392
The development of recombinant bacteria for insect pest control. (2000)
Neil Crickmore.
Memorias Sociedad Colombiana de Entomologia 27, 73-86
The diversity of Bacillus thuringiensis delta-endotoxins. (2000)
Neil Crickmore.
in "Entomopathogenic bacteria : from laboratory to field application". Klewer
Academic Publishers, London Eds J-F Charles, A Delecluse and C Nelson-LeRoux
pp 65-79
