The Sussex Centre for Advanced Microscopy

Microscope Training  Instructions  Camera   Booking      Sample preparation  Methodologies  Associated instrumentation 

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Contract Work

Research

Publications

Research example: AD and the Tauopathies website

Background  to EM

Interesting EM and other scientific links

Compiled by: Julian Thorpe

Other Instrumentation 

(funded by )

ultramicrotome ; CCD camera ; cryo-preparation system ; cryo-transfer sytem ; freeze-substitution apparatus

(a)  a Leica Ultracut Ultramicrotome with cryo-sectioning capability: for cutting of routine and cryo thin sections for examination in the TEM

(b)  an axially-mounted Gatan Ultrascan 1000 CCD camera: for the acquisition of 2K X 2K pixel, 16 bit digital image output from the TEM

(c)  a Leica EM CPC Cryo-Preparation System: for the cryo-fixation of cells, tissues and particulate samples on TEM grids.

(d)  a Gatan CT3500 Cryo-Transfer System: for cryo-transfer of particulate samples on TEM grids for examination by cryo-TEM

(e)  a Leica AFS Freeze-Subsitution System: for low temperature freeze-substitution and resin-embedding of samples