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The Process of Immunogold Labelling
N.B. This sequence of diagrams illustrates double immunogold labelling utilising antibodies raised in two different donor species. The first antibody immunolabelling steps shown are equivalent to a single-labelling experiment. THIS IS BY FAR THE METHOD OF CHOICE FOR DOUBLE IMMUNOLABELLING - OTHER METHODS ARE OFTEN FRAUGHT WITH METHODOLOGICAL AND/OR IMMUNOLOGICAL PROBLEMS (see Introduction of Thorpe, 1992). Also please note that double-labellings such as this may be carried out by mixing the two primary antibodies and also the two secondary antibody-gold probes together (the two primary antibody incubations are shown sequentially here to ease visualisation of the process). For examples, see image gallery.
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