Literature: A-K ; L-Z ; subject area
Compiled by: Julian Thorpe
Immunogold-labelled Isolated Tau Filaments from a Cytoskeletal Fraction
Lower Mag Image ; Higher Mag Image ; Highest Mag Image
Tau from a cytoskeletal fraction of a CBD brain homogenate double immunogold-labelled for total tau (rabbit polyclonal; goat anti-rabbit IgG-5nm gold secondary probe) and p-Thr231 tau (monoclonal; goat anti-mouse IgG-10nm gold secondary probe)
Specimens prepared by one of my 3rd. Year project student, Sarah Hoey
(a) Lower magnification image showing virtual absence of background p-Thr231 labelling (larger gold particle size; original mag = 20,000X; bar = 100nm)
(b) Higher magnification image: note the precise alignment of the larger 10nm gold particles (p-Thr231 tau) along the tau filament (original mag = 50,000X; bar = 50nm)
(c) Highest magnification image: some greater detail of the structure of the tau filament is visible here (original magnification = 70,000X; bar = 50nm)
